The scope of the present work was to study the release of methylene blue from a composite material based on collagen and hydroxyapatite.

The obtaining of the composite material was achieved by co-precipitation of hydroxyapatite in the collagen matrix (at a pH of 10.5). Later, methylene blue was added dropwise because the composite material is intended to act as support for releasing methylene blue; the release of methylene blue ensuring the antiseptic nature of the material. 

The composites were then characterized by: Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) while the release process was monitored by UV-Vis spectroscopy.

The characterization of the collagen/hydroxyapatite composite material revealed a good mineralization of collagen matrix a high stability of the mineral phase, as well as the capacity to bind/absorb methylene blue.

From the point of view of the release process, the results showed a gradual release that was evidenced by UV-Vis spectroscopy, the release evolution being monitored at a wavelength of 663 nm, characteristic.

Therefore, studies suggest the use of collagen/hydroxyapatite composite material as support for tissular regeneration of bone tissue, methylene blue providing antiseptic properties.