The desired pharmacological activity with minimum side effects of different traditional chemical formulations is achieved by enabling the site-specific targeting and controlled release. In this study, magnetic diethylaminoethyl-cellulose (DEAE-Cellulose) has been prepared by magnetite nanoparticles deposition on the DEAE-Cellulose support. The primary method to analyze the surface modification was SEM. DEAE-Cellulose/Fe₃O₄ was loaded with different antibiotics and their antimicrobial efficacy against bacterial strains belonging to E. coli and S. aureus species were studied. DEAE-Cellulose/ Fe₃O₄/cephalosporins have been characterized by using FT-IR. Our study indicates that the materials of DEAE-Cellulose/Fe₃O₄/cephalosporins have generally increased the activity against tested strains, the antimicrobial effect being not significantly dependent of temperature treatment.