Most of the artefacts of cultural and / or historical  value are exposed to fungal attack often causing irreversible damage. The need for new methods of antifungal protection led to the development of a recipe based on hydroxyapatite and barium hydroxide, with promising results. We synthesized and analytical characterized (trough energy dispersive X-ray fluorescence, X-ray diffraction and Fourier transform infrared spectroscopy) several materials based on hydroxyapatite. The efficiency of the synthesized materials was evaluated by diluted inoculums on the culture media technique and a modified Kirby-Bauer method, using simulated artefacts, previously reported to replicate the real artefacts.