The purpose of this work was to create an in vitro model of tissue engineered hybrid biocompatible and bioresorbable composite by combining beta-tricalcium phosphate powders (β-TCP), as the main component, with collagen. Moreover, the collagen’s osteoinductive capacity is associated with the superior bioactivity and osteoconduction of the β-TCP. In the present study two variants of COL/β-TCP scaffolds (P1 and P2) were prepared and examined. Microcrystalline β-TCP powders were prepared at high temperatures by solid-state reactions, and the nano-powders were produced by a precipitation technique. The collagen-TCP sponges were obtained by the reaction of two sorts of β-TCP powders (micro and nanometric size powders) with type I collagen under the physiological conditions. The morphology and chemical composition were analyzed semiquantitatively using X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and infrared spectroscopy (IR). The biocompatibility of the materials was evaluated by cytotoxic effects (cell death and cell proliferation). The biocompatibility of the obtained scaffolds was tested by biochemical methods on human fibroblast cultures. The Student^’s t-test was used for all statistical analyses.